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  • Use of Petri Dish in the Screening and Cloning of Transfected Cells
    Post time: Nov-23-2021

    Use of Petri Dish in the Screening and Cloning of Transfected Cells 1. 48 hours after transfection, the cells were divided into a culture medium containing 125 μmol/L L-histidine and 0.5 μg/ml tetracycline-HCl using several dilutions. The cell density is approximately 1 × 106 to 3 × 104 per 10 cm...Read more »

  • Learn to grow and transfect cells
    Post time: Nov-18-2021

    Learn to grow and transfect cells pTet-tTAk stably transfects fibroblasts Culture and transfect cells 1. Cultivate adherent cells in DMEM complete culture medium. The day before transfection, the cells were changed to DMEM complete culture medium containing 0.5 μg/ml tetracycline-HCl (tetracyclin...Read more »

  • Let’s understand cell passaging together
    Post time: Nov-18-2021

    Let’s understand cell passaging together It is recommended to pass down the cells every 2 days. Overgrown cells will reduce the natural differentiation rate of cells. We have established a purification method to remove differentiated cells. Adhere to uncoated tissue culture plates to remove...Read more »

  • Serial dilution separation method
    Post time: Nov-18-2021

    Serial dilution separation method ① Take 1 gram of soil sample, put it in an Erlenmeyer flask containing 99 mg of sterile water beside the flame, shake it well to disperse the bacteria. ② Take 1 ml of the above bacterial suspension with a pipette, put it into a test tube containing 9 ml of steril...Read more »

  • Isolation of mouse mononuclear cells
    Post time: Nov-17-2021

    Isolation of mouse mononuclear cells Basic plan: Preparation of cell suspension from spleen, thymus and lymph nodes Experimental Materials: 1. RPMI-5 or DMEM-5 complete medium 2. Freshly isolated mouse organs: mouse thymus ≤ 6 weeks old; mouse spleen and lymph nodes from 6 weeks to 6 months old 3...Read more »

  • Rapid determination of seed germination rate—paper fluorescence method
    Post time: Nov-17-2021

    Rapid determination of seed germination rate—paper fluorescence method Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is...Read more »

  • Rapid determination of seed germination rate—Red ink staining method
    Post time: Nov-17-2021

    Rapid determination of seed germination rate—Red ink staining method   Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is...Read more »

  • Rapid determination of seed germination rate—BTB method
    Post time: Nov-17-2021

    Rapid determination of seed germination rate—BTB method    Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly ...Read more »

  • Rapid determination of seed germination rate—TTC method
    Post time: Nov-17-2021

    Rapid determination of seed germination rate—TTC method    Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly re...Read more »

  • Production and purification of cell culture transfected Wnt ligand
    Post time: Nov-15-2021

    Production and purification of cell culture transfected Wnt ligand [Essence summary] Wnt ligand protein is abundant in vitro, but it is very difficult to purify. This method uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect Wnt11r protein...Read more »

  • Operating Procedures for Embryonic Stem Cell Culture
    Post time: Nov-15-2021

    Operating Procedures for Embryonic Stem Cell Culture [ different size petri dish,dish petri,disposable sterile petri dish,15cm petri dish,sterile plastic petri dishes ] Maintain ES cells in an undifferentiated state: ES cell culture uses high-sugar medium containing ESGRO (leukemia inhibitory fac...Read more »

  • Use of Petri Dish in Bioassay of Cytokinin
    Post time: Nov-15-2021

    Use of Petri Dish in Bioassay of Cytokinin [ cheap petri dish,compartment petri dish,sterile petri dishes,petri dish 4 ] Biological identification of cytokinin (radish cotyledon weight gain method) [Principle] Cytokinin has the effect of promoting the enlargement of radish cotyledons (increase in...Read more »

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