Rapid determination of seed germination rate—TTC method
Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly related to the amount of seed used when sowing. However, the conventional method (direct germination) requires a long time to determine the germination rate, especially for emergency needs, there is not enough time to determine the germination rate, and it is impossible to know when it encounters dormant seeds. The rapid determination method can obtain the result in a short time.
Triphenyltetrazolium chloride method (TTC method)
Principle: All seed embryos with vitality have redox reactions during respiration, while seed embryos with inanimate omissions do not have this reaction. When TTC penetrates into the living cells of the seed embryo and acts as a hydrogen receptor, it is reduced by the hydrogen on the dehydrogenation coenzyme (NADH2 or NADPH2), and changes from colorless TTC to red triphenylformaldehyde (TTF).petri dish 90mm,sterile petri dishes,petri dish 90mm sterile,15cm petri dish,plastic petri dish
Instruments and drugs: incubator, beaker, petri dish, tweezers, blades, balance, 0.5% TTC solution (weigh 0.5g TTC in a beaker, add a little 95% ethanol to dissolve it, and then dilute to 100ml with distilled water. Just keep it, if it turns red, it can’t be used again.)
Steps:
1. Seed soaking
Soak the tested seeds in 30-35℃ warm water (barley, wheat, indica for 6-8 hours, corn for about 5 hours, and japonica for 2 hours) to enhance the respiration strength of the embryo and make the color development fast.petri dish 90mm,sterile petri dishes,petri dish 90mm sterile,15cm petri dish,plastic petri dish
2. Color rendering
Take 200 swollen seeds, cut them into two halves along the center line of the seed embryo with a blade, put half of them in two petri dishes, each with 100 halves, and add an appropriate amount of 0.5% TTC to cover The seeds are degrees. Then put it in a thermostat at 30°C for 0.5-1 hour. As a result of observation, the embryos that are dyed red are live seeds.
The other half was boiled in boiling water for 5 minutes to kill the embryos, and the same staining treatment was performed for observation as a control.
3. Calculate the percentage of live seeds and compare it with the actual germination rate if possible to see if it matches.petri dish 90mm,sterile petri dishes,petri dish 90mm sterile,15cm petri dish,plastic petri dish
Post time: Nov-17-2021