Comparison of fluorescence PCR and gold labeling in the detection of Chlamydia trachomatis

Comparison of fluorescence PCR and gold labeling in the detection of Chlamydia trachomatis

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The prominent clinical symptoms of male infection with Chlamydia trachomatis are epididymitis and prostatitis. The main symptoms of female infection are disc inflammatory disease, which in turn causes salpingitis. Pregnant women infected with this pathogen will cause infertility, premature delivery, miscarriage and stillbirth, which can be seen Chlamydia trachomatis seriously affects the level of human reproduction. Since Chlamydia trachomatis poses such a big threat to our health, what effective detection methods should we adopt for identification? For this reason, the author made a comparison of the two main detection methods, hoping to help people choose the appropriate method to ensure our health.
In the detection methods of Chlamydia trachomatis, there are mainly fluorescent PCR method and gold labeling method. Both methods have their own characteristics. Please see the detailed description below.

The fluorescent quantitative PCR method, as its name implies, can be both qualitative and quantitative. It mainly detects the amount of template in the sample by detecting the accumulation of fluorescence during the PCR process. The amount of template in the sample can be obtained by direct quantitative analysis. . The fluorescent quantitative PCR method has relatively high technical requirements for experimental equipment and equipment, but it has outstanding advantages such as rapidity, high sensitivity, and strong specificity. However, because it can also detect positive for dead Chlamydia trachomatis, it is clinically It is prone to false positives, which is not of great significance to clinical treatment.

The so-called gold labeling method is the method of using immunogold/selenium rapid experiment. The reagents used in this method are relatively stable and can be stored for a long time at room temperature. The detection speed is high and the patient can get the results quickly. However, the accuracy of this method is too low, the misdiagnosis rate is high, and treatment is easily delayed, causing sequelae.