Comparison of the usage of various boards in the laboratory

Comparison of the usage of various boards in the laboratory

1. ELISA plate
The microplate is a kind of plate that is equipped with microplate reader for enzyme-linked immunoassay. The commonly used one is 96-well, which is mainly designed to cooperate with microplate reader. There are also 48-well plates, but they are not commonly used. In enzyme-linked immunoassays, antigens, antibodies and other biomolecules are adsorbed to the surface of the enzyme-labeled plate through a variety of mechanisms, and then the test sample and the enzyme-labeled antigen or antibody are reacted in different steps, and the detection is carried out by the microplate reader. .
2. Culture plate
The culture plate is used to culture cells or bacteria. There are 6 holes, 12 holes, 24 holes, 48 ​​holes, and 96 holes. It looks like a transparent ELISA plate, but its use is quite different. Add an appropriate amount of culture medium to the wells of the culture plate, and then conduct cell culture in a suitable environment. General culture plates are flat-bottomed, suitable for suspension culture of cells and tissues, in addition to U-shaped bottom and V-shaped bottom. After surface modification treatment, it can make it have cell adherence culture and growth performance.
3. PCR board
The PCR plate is used in the PCR machine. It is the same as the microplate with the microplate reader. It is used as a solid-phase carrier for the sample to carry out the PCR reaction in it, and then the PCR machine is used for detection. In fact, to put it simply, a PCR plate is a combination of many PCR tubes, generally 96 wells.
4. Deep hole plate
Microplates, PCR plates, etc. can become microplates. Because the volume of each well is small, there is also a kind of plate in the laboratory that has relatively deep holes. Generally, the bottom is U-bottomed and made of polymer materials. With good chemical compatibility, it can be used for the storage of most polar organic solutions, acidic and alkaline solutions and other laboratory liquids.
5. Serum plate
It is made of transparent high-molecular polystyrene material, and the surface has been specially treated. It is mainly used for serum dilution, protein and antigen-antibody concentration determination.