How to choose the position of PCR tube (96-well PCR instrument)

How to choose the position of PCR tube (96-well PCR instrument)

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When we do a small number of PCR reactions on a 96-well PCR machine, the choice of position is always a problem, and most users will choose the middle well. It’s not scientific.

We choose the position of the PCR tube in order to choose the most accurate position of the temperature. The most accurate position of the temperature should be the position above the temperature sensor under the module (not considering the problem of inaccurate temperature of the sensor). The position of the temperature sensor of different PCR machines is different. For example, AB 2720 has only one in the middle, MJ 200 has three sensors, which are lower left, middle and upper right. Eppendorf should be in row A or H, while Dongshenglong 811 has three temperature sensors. Choose B1-2. , C1-2, C6-7, D6-7, B11-12, C11-12, because these points are temperature punctual.

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Post time: Oct-18-2021

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