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  • Microbial inoculation operation
    Post time: Apr-19-2022

    Microbial inoculation operation 1. Slant inoculation (with Staphylococcus aureus) (1) Before operation, wipe your hands with 75% alcohol, and light the alcohol lamp after the alcohol evaporates. (2) Hold the strain tube and the inclined plane between the thumb of the left hand and the other four ...Read more »

  • How to use the inoculation loop?
    Post time: Apr-14-2022

    How to use the inoculation loop? The inoculation loop must be sterilized with an infrared sterilizer before and after each use. That is to say, it is thoroughly burned once in the infrared sterilizer, and the metal rod or glass rod in the cavity of the infrared sterilizer must also be rotated. Af...Read more »

  • Basic Conditions for Bacterial Culture: Basic Equipment and Utensils
    Post time: Apr-13-2022

    Basic Conditions for Bacterial Culture: Basic Equipment and Utensils The equipment and appliances that must be available in the bacterial laboratory include: Incubator, C02 incubator, anaerobic cultivation equipment for bacterial culture; Microscopes for observing bacterial morphology and direct ...Read more »

  • Bacterial inoculation method selection and comparison
    Post time: Apr-12-2022

    Bacterial inoculation method selection and comparison There are many inoculation methods for bacteria, such as streak method, coating method, pouring method, slant inoculation method, liquid culture medium inoculation method, spiral inoculation method, etc. The methods and applications are differ...Read more »

  • Inoculation loop operation steps
    Post time: Apr-11-2022

    Inoculation loop operation steps The prepared plate can be stored for 2-5 days when the inoculation loop is stored, and direct light exposure should be avoided. The dry medium should be placed in a dark and dry place, and the storage temperature should be 2-8°C. Expired dry media should not be us...Read more »

  • What actions can reduce aerosol generation?
    Post time: Apr-08-2022

    What actions can reduce aerosol generation? 1. In order to prevent the hot inoculation loops from being placed in the bacterial solution to generate aerosols, two inoculation loops can be used alternately. 2. When mixing the microbial suspension, use rotary rotation instead of left and right shak...Read more »

  • We must understand what are the operations that generate aerosols?
    Post time: Apr-06-2022

    We must understand what are the operations that generate aerosols? 1. Mild (<10 particles): glass slide agglutination test; pouring venom; burning the inoculation loop on a flame; extracting the culture medium. 2. Moderate (11 to 100 particles): inject, pour, and suspend bacterial solution bef...Read more »

  • Why must the bacterial culture application form indicate the source of the specimen?
    Post time: Mar-28-2022

    Why must the bacterial culture application form indicate the source of the specimen? The main work of the clinical microbiology laboratory is to isolate and accurately identify pathogenic bacteria from clinical specimens, and at the same time guide the rational clinical application of drugs, so a...Read more »

  • Experiment of slant medium inoculation method
    Post time: Mar-25-2022

    Experiment of slant medium inoculation method This method is suitable for pure culture and preservation of strains, and can also be used for the identification test of urea and Krebsaccharide medium. The method of inoculation is slightly different due to different purposes. The slant inoculation ...Read more »

  • The effect of chemical factors on bacteria
    Post time: Mar-24-2022

    The effect of chemical factors on bacteria (sterilization test of chemical disinfectants) 【Material】 1. Bacteria: Staphylococcus agar slant culture for 18-24 hours. 2. Medium: ordinary agar plate. 3. Chemical disinfectant: 5% carbolic acid, 2% iodine, 70% alcohol, 0.1% mercury chloride. 4. Othe...Read more »

  • How to cultivate bacteria
    Post time: Mar-23-2022

    How to cultivate bacteria [Culture in liquid medium] ⑴ Take 5ml of liquid culture medium into a sterile test tube. (2) Pick a single colony with an inoculation loop or a sterilized toothpick and inoculate it into the culture medium. (3) Cover the test tube and incubate overnight at 37°C on a shak...Read more »

  • Control culture of Escherichia coli, isolation of single colony and preservation of strains
    Post time: Mar-22-2022

    Control culture of Escherichia coli, isolation of single colony and preservation of strains Escherichia coli is a receptor bacterium widely used in molecular genetics experiments and genetic engineering operations. Its genetic background has been studied in detail, and it is often used as a host ...Read more »

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