How to inoculate a liquid medium Inoculation Loop And Needle,Disposable Inoculating Loops,Inoculating-Loop When inoculating a small amount of bacteria into the meat extract peptone liquid medium, the operation steps are basically the same as those of the inclined surface inoculation, the differen...Read more »

How microorganisms are inoculated on a slant (1) On the slanted test tube of meat extract peptone, use a marker to write the name of the bacteria to be inoculated, the date and the inoculated person. (2) Light an alcohol lamp or a gas lamp. (3) Hold the strain test tube and the inclined test tube...Read more »

Why aseptic inoculation technique is important The culture characteristics of microorganisms refer to the population morphology and growth of microorganisms cultured on the medium. Generally, slant, liquid and semi-solid media can be used to test the culture characteristics of different microorga...Read more »

What is the difference between culture flasks and a petri dishes? Difference Between Culture Flasks and Petri Dish The culture flasks are suitable for long-term culture, passage, and seed cells. Not easy to contaminate. Petri dishes and culture plates are suitable for use in various experiments...Read more »

Determination of Antibiotic Potency by Tube and Disk Method [Purpose and Requirements] 1. Understand the principle of the tube-dish method. 2. Master the technique and calculation method of the two-dose method to determine the antibiotic titer unit. [Principle] The bioassay of antibiotics is ...Read more »

Culture of rat aortic endothelial cells Material : Petri dish, culture flask, flask, test tube, glass needle, etc. Ophthalmic scissors, ophthalmic forceps, surgical blades. 5% CO2 incubator, PH meter. Constant temperature water bath. PMi1640 medium, D-Hank’s buffer, fetal bovine serum, endo...Read more »

Method for preparing villous chromosomes The villi are derived from the mesoderm of the embryo. The earliest primary dry villi appear at the beginning of the third week of gestation, and about 8 weeks of gestation is the most vigorous period of villi development. At present, the fluff materials a...Read more »

Layer plating method First pour a layer of basic medium without bacteria in the petri dish, after condensation, add a layer of basic medium containing bacteria; after condensation, add a layer of basic medium without bacteria, after the bacteria appear after culture , mark the corresponding posit...Read more »

PEI transfection method Formula: PEI stock solution (100 μM): Weigh 125 mg of PEI powder, dissolve it in 50 ml of 1×HBS (pH 7.4), filter through a 0.2 μm filter, and store at 4°C for later use. 1×HBS (pH 7.4): Dissolve 8.76 g NaCl in 900 ml ultrapure water, add 20 ml 1 M HEPES, adjust the pH to...Read more »

Culture of normal rat aortic endothelial cells Experimental Materials: 1. Rat aortic vessels; 2. 1×PBS without Ca2+ and Mg2+, add 200000IU/L penicillin, 200mg/L streptomycin, pH7.2; 3. Culture medium: M199 medium or RPMI1640 medium (containing 20% ​​calf serum, pH 7.2); 0.125% trypsin-0.01% ED...Read more »

Calcium phosphate transfection 1. Cell plate: Collect cells by trypsinization and plate cells on a 60 mm tissue culture dish at a density of 1 × 105 to 4 × 105 cells/cm² with appropriate complete medium (choose the culture dish according to the experimental needs, The area occupied by the cells a...Read more »

Observation on the preservative effect of preservatives Liquid pharmaceuticals are easily contaminated by microorganisms, especially aqueous liquid pharmaceuticals containing nutritious substances such as sugars, proteins, etc., which are more likely to cause the growth and reproduction of microo...Read more »