Rapid determination of seed germination rate—paper fluorescence method

Rapid determination of seed germination rate—paper fluorescence method

Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly related to the amount of seed used when sowing. However, the conventional method (direct germination) requires a long time to determine the germination rate, especially for emergency needs, there is not enough time to determine the germination rate, and it is impossible to know when it encounters dormant seeds. The rapid determination method can obtain the result in a short time.
Fluorescence on paper
Principle: The seed coats of viable seeds and dead seeds have different permeability to substances, and the seeds of many plants contain fluorescent substances. Using the different permeability of fluorescent substances to distinguish the life and death of seeds is simple, especially for the seeds of cruciferous plants.
Instruments and medicines: ultraviolet fluorescent lamps, petri dishes, tweezers, filter paper (no fluorescence)
Steps:
1. Soak 100 seeds of cruciferous plants such as rape, cabbage and other undamaged seeds in water at 25-30℃ for 2-3 hours.
2. The swollen seeds are neatly arranged on the wet filter paper in the petri dish at intervals of 3 to 5 mm. The water on the filter paper should not be too much to avoid the influence of the dispersion of fluorescent substances. It is not necessary to cover the petri dish, let it stand for 1.5-2 hours, remove the seeds, and dry the filter paper in the shade. The removed seeds are still arranged in another petri dish in the original order (for verification).
3. Place the dry filter paper under an ultraviolet fluorescent lamp for observation. If the observation can be carried out in a dark room, the effect will be better.90mm Petri Dish,Petri Dish 90mm,Petri Dish 90mm Sterile,Wholesale Petri Dish 90mm Sterile
4. If you see a fluorescent circle on the position where the seed is let go, it is a dead seed. If you want to confirm that they are dead seeds, you can pick out the seeds arranged in another petri dish and concentrate them on the wet filter paper of a petri dish, and leave the seeds that do not produce fluorescent rings in the petri dish to maintain humidity , Let it sprout naturally.
After 3-4 days, record the number of germinated seeds in the petri dish and fill in the table below.90mm Petri Dish,Petri Dish 90mm,Petri Dish 90mm Sterile,Wholesale Petri Dish 90mm Sterile
The success or failure of this method is firstly determined by the presence of fluorescent substances in the seeds, and secondly by the properties of the seed coat. Some seeds, whether they have the ability to germinate or not, once soaked, fluorescent substances will leak out. Soybeans fall into this category;
Due to the impermeability of the seed coat, some seeds do not produce fluorescent rings regardless of whether the seed is alive or dead. Many plant seeds will encounter this individual phenomenon. 90mm Petri Dish,Petri Dish 90mm,Petri Dish 90mm Sterile,Wholesale Petri Dish 90mm Sterile  At this time, the seed coat can only be re-verified by mechanically scratching the seed coat. On the contrary, sometimes due to damp when harvesting, the seed coat is broken, and a fluorescent ring will also be produced, so you should pay attention to it during the experiment. It is best to check the soaking liquid, and it is suitable for the test material if there is no fluorescence.90mm Petri Dish,Petri Dish 90mm,Petri Dish 90mm Sterile,Wholesale Petri Dish 90mm Sterile

Rapid determination of seed germination rate---paper fluorescence method


Post time: Nov-17-2021

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