Rapid determination of seed germination rate—Red ink staining method

Rapid determination of seed germination rate—Red ink staining method

  Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly related to the amount of seed used when sowing. 9cm Petri Dishes,types of petri dishes,different size petri dish,Petri Dishes Plastic Sterile.  However, the conventional method (direct germination) requires a long time to determine the germination rate, especially for emergency needs, there is not enough time to determine the germination rate, and it is impossible to know when it encounters dormant seeds. The rapid determination method can obtain the result in a short time.
Red ink staining method
Principle: The plasma membrane of living cells has the ability to selectively absorb substances, but the plasma membrane of dead germ cells loses this ability, so the dye can enter the dead cells and stain.
Instruments and drugs:
1. Seed soaking
Soak the tested seeds in 30-35℃ warm water (barley, wheat, indica for 6-8 hours, corn for about 5 hours, and japonica for 2 hours) to enhance the respiration strength of the embryo and make the color development fast.
2. dyeing
Take 200 swollen seeds and cut them in half along the midline of the embryo. Place half of them in a petri dish, add 5% red ink (submerged seeds as the degree), dye for 10 paws and 15 minutes (high temperature can be short some). After dyeing, pour out the red ink and rinse with water several times until the rinse liquid is colorless. Check whether the seeds are alive or dead. 9cm Petri Dishes,types of petri dishes,different size petri dish,Petri Dishes Plastic Sterile.  Those whose embryos are not colored or very lightly colored are live seeds; those whose embryos and endosperm have the same degree of coloring are dead seeds. Seeds killed by boiling water can be used as a control observation.9cm Petri Dishes,types of petri dishes,different size petri dish,Petri Dishes Plastic Sterile.

3. Count the number of uncolored or lightly colored seeds, and calculate the bud rate.

Rapid determination of seed germination rate---Red ink staining method


Post time: Nov-17-2021

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