Separation of petri dish plate by marking
① Take 1 gram of soil sample, add it to a conical bottle with 99 ml sterile water next to the flame, plug the cotton plug, and make bacterial suspension for use.Sterile Disposable Petri Dish,Sterile Plastic Petri Dish,Disposable Plastic Petri Dish
② Take a petri dish and place it on the experimental table. Open the dish slightly with your left hand and inject 10 to 12 ml of molten nutrient solid medium into the dish. Gently rotate the dish to make the medium evenly distributed, and put it flat on the table to form a condensed plate. Then divide zones A, B, C and D with crayons on the bottom of the dish. Several plates should be prepared consecutively.Sterile Disposable Petri Dish,Sterile Plastic Petri Dish,Disposable Plastic Petri Dish
③ Fix the bottom of the petri dish with thumb and ring finger into an inclined state, and open the dish slightly next to the flame.
At the same time, apply a small amount with ring vaccination needle near the flame bacteria suspension, quickly into the petri dish, on one side of the plate culture medium, for the first parallel line 6 ~ 7, turn about 70 ° Angle of petri dishes, with burnt cooling vaccination needle, through the first second underlined portion for parallel line, and then use the same method, a parallel line for the third time.
When marking, the surface of the plate medium should be downward to avoid airborne bacteria. The needle should be at an Angle of about 30° from the surface of the plate. Do not touch the edge of the dish with the inoculation needle and do not cut the medium.
(4) The petri dish was placed upside down at a warm place about 28℃ for culture. After the colony grew, the microbial group was identified, and according to the microscopic examination results, whether the pure bacterial species had been isolated. If the strain is pure, it can be transferred to slant medium for further culture. Continuous dilution separation and plate marking must be performed in a sterile room or inoculation box.
Post time: Jun-23-2022