Use of Petri Dish in Bioassay of Cytokinin

Use of Petri Dish in Bioassay of Cytokinin

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Biological identification of cytokinin (radish cotyledon weight gain method)
[Principle]
Cytokinin has the effect of promoting the enlargement of radish cotyledons (increase in fresh weight). The relationship curve between the cytokinin concentration and the weight increase of the cotyledon can be drawn first, and then the test sample can be used for a comparative test to identify the concentration or titer of the cytokinin in the sample.
[Instruments and Utensils]
5 sets of petri dishes (diameter 9cm); 5 round filter papers; 1 enamel dish (with lid); 1 tweezers; 1 surgical scissors.[ cheap petri dish,compartment petri dish,sterile petri dishes,petri dish 4 ]
[Reagent]
The right amount of bleaching powder;
5mg/L kinetin or 6-BA solution.
[Method]
1. Seed germination Take 200 uniform radish seeds, sterilize them with saturated bleaching powder solution for 20 minutes and wash them, place them in an enamel tray with filter paper and moistened with distilled water, and germinate in the dark at 25°C. About 30 hours later, two cotyledons, one large and one small, will germinate. Cut a smaller cotyledon from each seedling, taking care that no hypocotyls remain on the cotyledons. Choose 50 cotyledons of the same size for testing.[ cheap petri dish,compartment petri dish,sterile petri dishes,petri dish 4 ]
2. Cotyledon culture Take 5 sets of numbered petri dishes with a diameter of 9CM, and place a filter paper of the same size as the bottom of each petri dish, add 5.0, 0.5, 0.05, 0.005 mg/L to petri dishes from No. 1 to No. 5 respectively. 3ml each of kinetin or 6-BA aqueous solution and distilled water. The solution dilution method is the same as IAA, put 10 cotyledons on the filter paper of each petri dish, cover the dish, and place the petri dish on an enamel dish with moist filter paper, cover the dish with a glass plate to maintain high humidity , Cultivate for 3 days at 25°C under fluorescent light, weigh the fresh weight of each cotyledon with an analytical balance, and find the average.[ cheap petri dish,compartment petri dish,sterile petri dishes,petri dish 4 ]
3. Using the average weight of each treated cotyledon as the ordinate, and the logarithm of the kinetin or 6-BA concentration as the abscissa, draw the relationship curve between the two. Using the same method to culture radish cotyledons with an unknown sample solution and comparing with the standard curve, the content or biological potency of cytokinin in the unknown sample can be roughly determined.
[Note]
When analyzing unknown samples, it needs to be purified to remove the sugar and inorganic salts in the plant materials to prevent the samples from becoming moldy during the culture.

https://www.huidalab.com/eo-sterlization-various-sizes-petri-dish-with-or-without-vents-product/


Post time: Nov-15-2021

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