What are the inoculation tools and methods?

What are the inoculation tools and methods?

[Inoculation]
The process of attaching a microorganism to an artificial medium or living organism suitable for its growth and reproduction is called inoculation.
1. Inoculation tools and methods
In laboratory or factory practice, the most commonly used inoculation tools are inoculation loops and inoculation needles. Due to different inoculation requirements or methods, the tip of the inoculation needle is often made into different shapes, including knife-shaped and rake-shaped. When the bacterial liquid is to be uniformly coated on the surface of the solid medium, a coating rod is required. (As shown below)

1. Inoculation needle 2. Inoculation loop 3. Inoculation hook 4.5. Glass coated rod 6. Inoculation circle 7. Inoculation hoe 8. Small scalpel

1. Inoculation needle   2. Inoculation loop   3. Inoculation hook   4~5. Glass coated rod   6. Inoculation circle   7. Inoculation hoe   8. Small scalpel

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Commonly used inoculation methods are as follows:

1) Streak inoculation: This is the most commonly used inoculation method. That is, by moving back and forth linearly on the surface of the solid medium, inoculation can be achieved. Commonly used inoculation tools include inoculation loops, inoculation needles, etc. This method is commonly used in slanted inoculation and plate streaking.

2) Three-point inoculation: This method is commonly used in the study of mold morphology. This method is to inoculate a small amount of microorganisms on the surface of the plate to form three points of an equilateral triangle, and let them independently form colonies to observe and study their morphology. In addition to three points, there are also one or more points for vaccination.

3) Puncture inoculation: This method is often used when preserving anaerobic strains or studying the kinetics of microorganisms. When doing puncture inoculation, the inoculation tool used is the inoculation needle. The medium used is generally a semi-solid medium. Its method is: dip a small amount of bacteria with an inoculation needle, and make a straight line puncture along the center of the semi-solid medium to the bottom of the tube. If a bacteria has flagella and can move, it can grow around the puncture line.

4) Pour and mix inoculation: This method is to put the microorganisms to be received into the petri dish first, then pour into the solid medium cooled to about 45 ° C, and shake it quickly and gently, so that the bacterial liquid can achieve the purpose of dilution . After the plate is solidified, it can be cultured at a suitable temperature to grow a single microbial colony.

5) Coating inoculation: It is slightly different from pouring and mixing inoculation, that is, pour the plate first, let it solidify, then pour the bacterial liquid on the plate, and quickly use the coating rod to coat the surface back and forth to let the bacterial liquid. Evenly distributed, you can grow a single colony of microorganisms.

6) Liquid inoculation: Wash the bacteria from the solid medium and pour it into the liquid medium, or from the liquid culture, use a pipette to connect the bacteria into the liquid medium, or from the liquid culture. Bacterial liquid is transferred to solid medium, which can be called liquid inoculation.

7) Injection vaccination: This method is to transfer the microorganisms to be received into living organisms, such as humans or other animals, by injection. some diseases.

8) Live inoculation: Live inoculation is a method specially used to cultivate viruses or other pathogenic microorganisms, because viruses must be inoculated in living organisms to grow and reproduce. The living body used can be the whole animal; it can also be a certain in vitro living tissue, such as monkey kidney; it can also be the developing chicken embryo. The way of inoculation is injection, it can also be mixed feeding.

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Post time: Mar-15-2022

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