What is the difference between the drum cover and the flat cover of the PCR tube

What is the difference between the drum cover and the flat cover of the PCR tube

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In early PCR, it is said that the drum cover design can better prevent evaporation. But then thermal-lid PCR machines appeared, and flat-lid PCR tubes were often used, and the drum-lid was inconvenient to use thermal lids.
The round ones are protruding, and the moisture will evaporate to the top of the lid after high temperature.Because the protuberances have slopes, the moisture will flow down along the trend and flow into the tube.
For flat caps, one is because sometimes the cap is not tight, and the other is that after the water evaporates to the top of the cap, it will stay in the cap, and if it accumulates, it will flow out from the side and flow out of the tube. If you want to verify, you can make a comparison .After the PCR is done, measure the volume in the two cases.
Which one to use mainly depends on the PCR machine.
Current PCR machines have thermal covers, but they are divided into two types, flat and drum, corresponding to two types of PCR tubes.

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Post time: Oct-18-2021

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