-
Bacteria isolation and inoculation method Bacterial isolation and inoculation is an important step in artificial isolation and culture of bacteria. At the same time, it is of great significance in the research of bacteriology, the preparation of biological products such as vaccines, toxoids, anti...Read more »
-
Gluconium ammonium medium [production method] First dissolve salts and sugars in water, adjust pH, add agar, heat to dissolve, then add indicator, mix evenly, divide into test tubes, sterilize at 121°C for 15 minutes, and place on a slope. [Experiment method] Gently touch the surface of the cultu...Read more »
-
Influence of biological factors on microorganisms (antibacterial spectrum test) [Experimental reagent] Bean sprouts juice Glucose agar medium [Laboratory equipment] Sterile petri dishes, inoculation loops, etc. Types Of Petri Dishes,Disposable Petri Dish,Plastic Inoculation Loop,Inoculation Lo...Read more »
-
Phage isolation steps The steps of the phage isolation process corresponding to some strains: 1. Phage isolation (1) Add 4g of fresh pigeon dung to 100ml of ordinary liquid medium, and put it into a 37 ℃ incubator for 24h. (2) The next day, take out 10ml of it and heat it at 70°C for 30min, centr...Read more »
-
What are the inoculation tools and methods? [Inoculation] The process of attaching a microorganism to an artificial medium or living organism suitable for its growth and reproduction is called inoculation. 1. Inoculation tools and methods In laboratory or factory practice, the most commonly used ...Read more »
-
Liquid culture medium transfer technology For the enrichment of pure bacteria and to observe the growth characteristics of bacteria in a liquid environment (cloudy growth, surface growth or sedimentary growth) (1)Material broth medium Slant cultures (Escherichia coli, Streptococcus pyogenes, Baci...Read more »
-
Cultivation of overnight suspension culture Instruments: alcohol lamp, inoculation loop Pharmaceutical reagents: E. coli strain (JM109) LB broth: 1% (w/v) Bacto tryptone, 0.5% Bacto yeast extract, 1% NaCl, adjusted to pH 7.0 with 5 N NaOH, then sterilized by moist heat. LB agar plates: LB medium ...Read more »
-
Preparation before inoculation (1) The inoculation room should be kept clean, scrub the table and walls with pulverized coal phenol soap, and regularly fumigate with lactic acid or formaldehyde. It should be sterilized by UV light before each use. Regularly check the sterility of the inoculation ...Read more »
-
Microbial isolation procedures Plate Scribing Separation The plate streaking method is a method in which the inoculation loop is streaked on the surface of the plate medium to achieve the separation of microorganisms. The principle is to dilute the microbial samples on the surface of the solid me...Read more »
-
Slant inoculation (with Staphylococcus aureus) (1) Before operation, wipe your hands with 75% alcohol, and light the alcohol lamp after the alcohol evaporates. (2) Hold the strain tube and the inclined plane between the thumb of the left hand and the other four fingers, so that the inclined plane...Read more »
-
Procedure for Negative Fungal Cultures 1. Carefully check whether the label and form of the specimen are correct, and whether the quality of the specimen is up to standard. If the received specimen is checked correctly with the sheet, and the quality of the specimen is up to standard, it will be ...Read more »
-
Slant culture medium transfer technique (1)Material Agar slant medium Isolated cultured plate cultures. (2) Method ①Indicate the name (or serial number) of the bacteria to be transplanted, the date of inoculation, the group and code of the inoculated person on the upper part of the test tube ...Read more »
