Absorbance test of pipette tips
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The accuracy and precision of pipetting not only require the matching of the tip and the pipette, but also a tip suitable for the liquid. [ Pipette Tip 1250,Pipette Tips 10ul,Pipette Tips Blue,Glison Pipette Tips,Pipette Tip Pipette ]When using a standard pipette tip (PP) to pipette liquids with low surface tension, it is easy to leave a film on the surface of the pipette tip (such as liquids containing detergents). [ Pipette Tip 1250,Pipette Tips 10ul,Pipette Tips Blue,Glison Pipette Tips,Pipette Tip Pipette ]The residual liquid will lead to inaccurate and inconsistent pipetting results, and at the same time some expensive samples will be lost. The reagents and samples used in many DNA and protein analysis methods usually contain detergents. The development of low-absorption tips is to improve the common problem of liquid residue.
Absorbance test of pipette tips
In the absorbance test, we use a staining reagent (green food dye dissolved in distilled water) to determine the residual liquid in the pipette tip after discharge. Use the maximum nominal volume of the test tip to draw the green reagent. Then drain the liquid directly back into the container. Next, rinse the tip with distilled water at the maximum capacity of the tip 5 times. Then use a spectrophotometer (405 nm) to measure the absorbance of the rinse solution and compare the result with the reference reagent. The absorbance of the rinse liquid is directly related to the amount of liquid remaining in the tip.[ Pipette Tip 1250,Pipette Tips 10ul,Pipette Tips Blue,Glison Pipette Tips,Pipette Tip Pipette ]
Post time: Oct-21-2021