For different types of liquids, pipettes have different cleaning and maintenance methods
| Liquid to be processed | Prevention method | Cleaning and maintenance methods |
| Aqueous solution and buffer | Disassemble the lower end of the pipette, rinse the contaminated parts with double distilled water, dry under 60℃ or air dry, and apply a small amount of grease to the piston | |
| Inorganic acid or base | =Use pipette tip with filter | After pipetting, remove the lower end of the pipette and place it in a ventilated place. If necessary, use double distilled water to rinse the contaminated parts, dry them below 60℃ or air dry, and apply a small amount of grease to the piston |
| Infectious fluid | =Use pipette tip with filter = Forward pipetting | = Forward pipetting Remove the lower end of the pipette, soak it in the laboratory’s conventional disinfectant for 30 minutes, then wash it with double distilled water, and autoclave the contaminated parts at 121°C, 1 bar, and 20 minutes |
| Cell culture | =Use pipette tip with filter | = Forward pipetting Remove the lower end of the pipette, soak it in the laboratory’s conventional disinfectant for 30 minutes, then wash it with double distilled water, and autoclave the contaminated parts at 121°C, 1 bar, and 20 minutes |
| Organic solvents | = Forward pipetting | After pipetting, remove the lower end of the pipette and place it in a ventilated place. If necessary, use double distilled water to rinse the contaminated parts, dry them below 60℃ or air dry, and apply a small amount of grease to the piston |
| Radioactive solution | =Use pipette tip with filter = Forward pipetting | Remove the lower end of the pipette, soak the contaminated parts in a composite solution or a special cleaning solution for 30 minutes, then wash with double distilled water, dry at 60℃ or air dry, and apply a small amount of lubrication to the piston fat. |
| Nucleic acid or protein solution | =Use pipette tip with filter = Forward pipetting | Nucleic acid: Boil in aminoacetic acid or hydrochloric acid buffer (pH2.0) for 10 minutes, rinse with double distilled water, dry at 60℃ or dry naturally, and apply a small amount of grease to the pistonProtein: Remove the lower end of the pipette, clean it with detergent, rinse with double distilled water, dry at 60℃ or dry naturally |
Post time: Oct-27-2021