Gravimetric analysis of the amount of liquid remaining in the pipette tip
When pipetting liquids with low surface tension (such as reagents containing detergents, etc.), a liquid film that is imperceptible to the naked eye is usually left on the inner wall of the pipette tip. The presence of this liquid residue will cause inconsistent and inaccurate pipetting results, and cause the loss of precious samples.[ Pipette Tips With Filter,200ul Gilson Filter Tips,Sterile Tips With Filter,Filter Pipette Tip 200ul,Pippete Tips With Filter ]
In recent years, various brands have launched low-absorption tips, hoping to minimize the impact of this kind of residue on the experiment. The following uses low-absorption tips manufactured by different suppliers to treat liquids containing detergents, observe the residual amount of liquid in the tips, and compare the performance of low-absorption tips.
Material
Standard tip: 200μ, 350μl
Low Retention tip: 200μl, 350μl
Low Retention filter tip: 120μl
Low Retention tips from other suppliers: 200μl, 300μl
Other suppliers’ Low Retention filter tips: 200μl
Electric pipette: 10-300μl, aspirate liquid at 4 speeds, and discharge liquid at 1 speed
Manual pipette: 20-200μl
Detergent solution: 0.1% Triton X-100, 10% Tween 20, 10% SDS, staining 10X PCR buffer (contains detergent, density reagent and tracking dye)
Microbalance, analytical balance BP211D
Gravimetric method
After pipetting, use gravimetric analysis to measure the amount of liquid remaining in the pipette tip. Place the small glass container filled with reagents on the balance and set the weight to zero. Then draw a certain amount of reagent from the container and release it back, and record the balance reading showing the amount of liquid remaining in the tip. Repeat this operation for each detergent solution listed above.[ Pipette Tips With Filter,200ul Gilson Filter Tips,Sterile Tips With Filter,Filter Pipette Tip 200ul,Pippete Tips With Filter ]
Post time: Oct-21-2021