How to inoculate a liquid medium
Inoculation Loop And Needle,Disposable Inoculating Loops,Inoculating-Loop
When inoculating a small amount of bacteria into the meat extract peptone liquid medium, the operation steps are basically the same as those of the inclined surface inoculation, the difference is that after the inoculation loop for picking the bacteria is placed in the liquid medium, it should be placed on the inner wall of the tube at the liquid surface. Gently rub on the top to make the cells fall off the ring, and mix them into the liquid medium. After plugging the test tube, shake the liquid to make the cells evenly distributed in the liquid, or mix with a test tube shaker.
When the amount of inoculum in the liquid medium is large or quantitative inoculation is required, sterile water or liquid medium can be injected into the strain test tube, scraped off the bacterial moss with an inoculation loop, and then the strain suspension is quantitatively aspirated with a sterile pipette and added. , or pour directly into liquid medium. If the strain is a liquid culture, it can be added by quantitative suction with a sterile pipette or directly poured into the liquid medium. The entire inoculation process requires aseptic manipulation.
Inoculation Loop And Needle,Disposable Inoculating Loops,Inoculating-Loop
Post time: Mar-02-2022