How to troubleshoot pipettes
| Phenomenon | Reason | Solution |
| Abnormal piston movement | = Piston is installed too tightly= Insufficient piston lubrication
= Solidification of the grease on the surface of the piston = Foreign matter or damage on the surface of the piston =There are impurities and particles between the piston, O-ring and the tip connecting rod = O-ring is damaged |
——Disassemble the lower end and reassemble——Grease the piston——Remove old grease and apply new grease
——Clean the piston or replace the piston ——Clean and lubricate the O-ring and the tip connecting rod ——Replace O-ring |
| Inaccurate pipetting volume or leakage | =Improper installation of the Pipette Tip = Pipette Tip does not match =There are foreign particles between the Pipette Tip and the Pipette Tip connecting rod = The Pipette Tip is damaged = Pipetting too fast = The Pipette Tip leaves the liquid level too fast= Foreign matter or damage on the surface of the piston =There are impurities and particles between the piston, O-ring and the Pipette Tip connecting rod = Insufficient lubrication of O-ring and piston = O-ring is damaged = Incorrect operation |
——Reinstall the Pipette Tip——Clean the tip connecting rod and replace with a suitable Pipette Tip ——Clean the tip connecting rod and replace the Pipette Tip ——Replace with a new Pipette Tip ——Adjust the pipetting speed, slow aspiration and slow release ——When pipetting a viscous, large-volume liquid, stay for a few seconds after aspirating the liquid, and then remove it from the liquid surface ——Clean the piston or replace the piston ——Clean and lubricate the O-ring and the tip connecting rod——Grease on ——Replace O-ring ——Please operate according to the instructions |
| Residual fluid in the tip Residual fluid in the tip | =The mode with additional liquid is selected(Reverse, sub-sample, unequal volume) = Pipette Tip does not match =Improper installation of the tip |
——Press the start button to empty the Pipette Tip ——Use the right Pipette Tip ——Reinstall the Pipette Tip |
| = High adsorption of the Pipette Tip= The liquid is too viscous | ——Replace the Low Retention Filter Tips ——Select the reverse pipetting mode, pre-wash the Pipette Tip, and slow down the suction and release speed |
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| Noise during operation | = Insufficient piston lubrication | ——Grease the piston |
| Inaccurate dispensing of special liquids | = Improper calibration, high-viscosity liquids need to be re-calibrated= Pipette liquids that are easily volatile or whose density is significantly different from water | ——Recalibrate with problem liquid——Pre-wash the tip or recalibrate |
| Tips fall off or are difficult to install | =Poor quality Pipette Tip=The Pipette Tip has been damaged | ——Use high-quality Pipette Tip——Replace with a new Pipette Tip |
Post time: Oct-27-2021