1. Smear method is a film making method that evenly spreads the material on the slide.
Smear material has single cell organism, small algae, blood, bacterial culture fluid, loose organization of animal and plant, testis, anther and so on.
Note when taking smear:
(1)The slide must be clean.
(2)Slides should be flat.
(3)The coating must be uniform. Apply the droplet to the right of the center of the slide and evenly apply it with a dissecting knife blade or toothpick.
(4)The coating should be thin. With another slide as a push slide, along the slide surface with smear drops (the Angle between two slides should be 30°-45°) from right to left gently push, coated into a uniform thin layer.
(5)Fixed. If necessary, chemical fixation or drying (bacterial) fixation can be used.
(6)Staining. Methylene blue is used for bacteria, Reye’s dye is used for blood, and sometimes iodine is used. The dye should cover the entire surface.
(7) flushing. Blot or bake dry with blotting paper.
(8) Seal . Long – term storage with Canada gum seal pieces.
2. Tablet compression method is a method of making tissue cells by placing the biological material between the slide and the cover plate and applying certain pressure.
3. The loading method is to make the biological material into glass specimens by integral sealing, which can be used to make temporary or permanent loading.
The loading materials are: tiny organisms such as chlamydomonas, amoeba, nematode; Hydra, the leaf epidermis of plants; Insect wing, foot, mouth organ, human oral epithelial cells, etc.
Attention should be paid to the packing method:
(1) When holding the slide, attention should be paid to the flat or placed on the platform. When dripping water, the amount of water should be appropriate, so as to be fully covered by the cover glass.
(2) The material should be unfolded with dissecting needles or forceps without overlapping and flattened out on the same plane.
(3) When putting the cover glass, slowly cover the water drop from one side to prevent bubbles from appearing.
(4) When dyeing, put a drop of dye liquid on one side of the cover glass and attract it from the other side with absorbent paper to make the specimen under the cover glass uniformly colored. After coloring, use the same method, drop a drop of water, the dye liquid sucked out, under the microscope to observe.
4. Sectioning method is a glass slide specimen made of thin slices cut from an organism.
Due to different requirements, the tissue can be sliced by hand with a blade, or the tissue can be embedded in paraffin or collodion, or frozen at low temperature, and sliced with a slicer. Cut into 5~10 micron slices for light microscope observation. Ultra-thin sections, with a thickness of 20~50 nanometers, made of epoxy resin or embedded tissue blocks of methacrylic acid, specially for electron microscope observation. General teaching with such as root tip, stem section commonly known as paraffin section.
Post time: Mar-30-2021