Layer plating method
First pour a layer of basic medium without bacteria in the petri dish, after condensation, add a layer of basic medium containing bacteria; after condensation, add a layer of basic medium without bacteria, after the bacteria appear after culture , mark the corresponding position on the bottom of the petri dish, and then add a layer of complete medium. The colonies that appear after re-cultivation are mostly auxotrophs that can only grow in the complete medium. The function of the upper and lower layers of the basic medium is to sandwich the colonies in the middle to prevent the bacteria from moving or being washed away by the complete medium.
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Post time: Feb-09-2022