Procedure for Negative Fungal Cultures

Procedure for Negative Fungal Cultures

1. Carefully check whether the label and form of the specimen are correct, and whether the quality of the specimen is up to standard. If the received specimen is checked correctly with the sheet, and the quality of the specimen is up to standard, it will be accepted. Otherwise, find the reason and record it in the relevant record book.

2. Specimen processing and result reporting:

1) The swab and secretion specimens were coated on TTC-Sabao plate to form the original line, and then separated by a sterile inoculating loop and then placed in a 25°C incubator for cultivation.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.

2) Take 5 urine samples from the urine, centrifuge at 3000 rpm for 5 minutes, take the centrifuged sediment, apply the sample in the inoculating loop to the TTC-Sabao plate to form the original line, and then separate it with a sterile inoculation loop and draw a line and set it at 25°C Incubator cultivation.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.

3) Centrifuge the drainage sample at 3000 rpm/centrifuge for 5 minutes, take the centrifuged sediment, apply the sample in the inoculating loop to the TTC-Sabao plate to form the original line, and then separate it with a sterile inoculating loop and draw a line, and then place it in a 25°C incubator for cultivation.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.

4) Sputum or pharyngeal swab specimens are sampled from suspicious parts of sterile inoculating loops and coated on TTC-SaPaulo plate to form an original line, then separated by sterile inoculating loops and then incubated in a 25°C incubator.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.

5) Intestinal specimens such as feces or anal swabs are taken with sterile inoculating loops to take suspicious parts and smear them on TTC-Saboro plate to form the original line, and then separate the lines with sterile inoculating loops and then culture in a 25°C incubator.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.

6) Indwelling needles, small catheters and other specimens are placed in sterile tubes, add enrichment solution 2, incubate at 35 °C for 48 hours to observe whether the culture solution is turbid, sample and apply it to TTC-SaPaulo plate to form the original line, and then use Sterile inoculating loops were separated by line drawing and then cultured in a 25°C incubator.

At the 24th, 48th, and 72nd hours of culture, observe whether there are suspicious colonies on the plate.