Separating Escherichia coli from drinking water with a petri dish
1. Make eosin methylene blue medium, pour it into a petri dish while it is hot, condense into a flat plate, and set aside.
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2. Use a sterilized Erlenmeyer flask to collect river water or ditch water, and dilute it at 1:10.
3. Take 0.1 ml of the dilution and inoculate it on the eosin blue medium. Separation is carried out by the flat scribing separation method.
4. Invert the streaked petri dish in a 37°C incubator and incubate for 18-24 hours. Colonies of Escherichia coli will appear in the medium, and the center of the colony is dark blue-black with metallic luster.Sterile Petri Dishes Wholesale,60 Mm Petri Dish,Price Petri Dishes,Petri Dish 90mm
5. Inoculate the colony on the slant medium (made from nutrient agar medium).
Post time: Nov-09-2021