Comparison of the usage of various plates in the laboratory

Comparison of the usage of various plates in the laboratory

1. ELISA plate
The ELISA plate is a plate used for enzyme-linked immunosorbent experiments on a microplate reader. The commonly used one is 96 wells, which are mainly designed to cooperate with the microplate reader. There are also 48 wells, but they are not commonly used. In the enzyme-linked immunosorbent assay, antigens, antibodies and other biomolecules are adsorbed to the surface of the enzyme-labeled plate through various mechanisms, and then react with the test sample and the enzyme-labeled antigen or antibody according to different steps, and are detected by a microplate reader. .
2. Culture Plates
The culture plate is used to culture cells or bacteria, and there are 6 wells, 12 wells, 24 wells, 48 ​​wells, and 96 wells. Similar to the transparent microplate, but the use is very different. Add an appropriate amount of culture medium to the wells of the culture plate, and then carry out cell culture in a suitable environment. General culture plates are flat-bottomed, suitable for suspension culture of cells and tissues, and there are also U-bottom and V-bottom. After surface modification treatment, it can have cell adherent culture and growth properties.
3. PCR plate
The PCR plate is used in the PCR instrument, just like the microplate plate is used with the microplate reader, it is used as a solid phase carrier, and the sample is subjected to PCR reaction in it, and then the PCR instrument is used for detection. In fact, to put it simply, a PCR plate is a combination of many PCR tubes, usually 96 wells.
4. Deep well plate
Like microplates, PCR plates, etc., they can become microplates, because the volume of each well is very small. There is also a plate in the laboratory, the hole is relatively deep, and the bottom is generally U-bottom, which is made of polymer materials. It has good chemical compatibility and can be used for the storage of most polar organic solutions, acidic and alkaline solutions and other laboratory liquids.
5. Serum Plate
It is made of transparent high-molecular polystyrene material, and the surface is specially treated. It is mainly used for serum dilution, protein and antigen-antibody concentration determination.