-
In vitro culture experiment of carrot root [ Plastic Petri Dish,Petri Dish 60mm,90mm Petri Dish ] 1. Rinse the carrots with tap water, remove 1-2mm of the skin with a paring knife, and cut into slices about 10-40mm thick. The following steps are all performed aseptically. 2. After the carrot sli...Read more »
-
Isolation of chloroplast pigments (1) Take a piece of circular qualitative filter paper (11cm in diameter) and poke a small circular hole (about 3mm in diameter) in its center. Take another filter paper strip (5cm×1.5cm), use a dropper to draw the ethanol chloroplast pigment extract and apply it ...Read more »
-
Oligodendrocytes are derived from rats aged 1-2 days. After decapitation, the rat brain was collected in Ham’s F-12 culture medium, and the meninges and blood vessels were removed. The cortex was homogenized by mechanical homogenization, and then the cell suspension was filtered through 230...Read more »
-
Experimental Materials: 1. Bone tissue source: newborn or embryonic rat, rabbit, human embryo or surgically excised bone tissue; 2. Washing solution: 1×PBS without Ca2+ and Mg2+, add 100IU/ml penicillin, 100μg/ml streptomycin, pH7.2; 3. Digestive solution: 0.25% trypsin solution, 1mg/ml type II c...Read more »
-
Experimental steps: 1. Put the sterile Microscope Slides Laboratory in the cell culture dish and take it out after the cells grow to semi-confluent. You can also choose to dye directly in a petri dish or a culture plate, and there is no need to put in a sterile Slide Glass Microscope at this tim...Read more »
-
[Method steps] 1 Take materials. In the clean workbench in the sterile room, take a bottle of culture at a time, burn about 20 mm from the mouth of the culture bottle, and use the tweezers and scalpel after burning to remove the explants or calluses. The tissue is placed in a sterile petri dish. ...Read more »
-
Colony counting method on petri dish (1) First calculate the average number of colonies at the same dilution. If one of the petri dishes has large sheet-like lawns, it should not be used. Instead, a petri dish without sheet-like lawns should be used as the average number of colonies for the dilut...Read more »
-
[Steps] 1. Water sample taking (1) Sterilize the tap water with a flame for 3 minutes, then open the tap to let the water flow for 5 minutes, and then take the water sample with a sterilized Erlenmeyer flask for analysis. (2) Pool water, river water or lake water should take a deep water sample 1...Read more »
-
Observation of Plant Root Exudates [Principle] The root system of a plant is an organ that is extremely active in life activities. It can synthesize some substances necessary for life, supply other organs, and at the same time excrete some substances from the body, changing the surrounding enviro...Read more »
-
Tropic movement of plants [principle] When plants are subject to certain stimuli, if the intensity of the stimulus from all directions is not uniform, they will produce movement. The phototropism and geotropism of plants are the tropism that is easy to be observed. The direction of movement is re...Read more »
-
Normal human breast epithelial cell culture 1. Collect milk 2-7d after delivery. Scrub the breast with sterile water and squeeze the milk into a sterile container. After mixing the collected milk, it is diluted with RPMI1640 to facilitate centrifugation. 2. Centrifuge the diluted milk (600-1000g,...Read more »
-
Use of Petri Dish in the Screening and Cloning of Transfected Cells 1. 48 hours after transfection, the cells were divided into a culture medium containing 125 μmol/L L-histidine and 0.5 μg/ml tetracycline-HCl using several dilutions. The cell density is approximately 1 × 106 to 3 × 104 per 10 cm...Read more »
