-
Learn to grow and transfect cells pTet-tTAk stably transfects fibroblasts Culture and transfect cells 1. Cultivate adherent cells in DMEM complete culture medium. The day before transfection, the cells were changed to DMEM complete culture medium containing 0.5 μg/ml tetracycline-HCl (tetracyclin...Read more »
-
Let’s understand cell passaging together It is recommended to pass down the cells every 2 days. Overgrown cells will reduce the natural differentiation rate of cells. We have established a purification method to remove differentiated cells. Adhere to uncoated tissue culture plates to remove...Read more »
-
Serial dilution separation method ① Take 1 gram of soil sample, put it in an Erlenmeyer flask containing 99 mg of sterile water beside the flame, shake it well to disperse the bacteria. ② Take 1 ml of the above bacterial suspension with a pipette, put it into a test tube containing 9 ml of steril...Read more »
-
Isolation of mouse mononuclear cells Basic plan: Preparation of cell suspension from spleen, thymus and lymph nodes Experimental Materials: 1. RPMI-5 or DMEM-5 complete medium 2. Freshly isolated mouse organs: mouse thymus ≤ 6 weeks old; mouse spleen and lymph nodes from 6 weeks to 6 months old 3...Read more »
-
Rapid determination of seed germination rate—paper fluorescence method Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is...Read more »
-
Rapid determination of seed germination rate—Red ink staining method Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is...Read more »
-
Rapid determination of seed germination rate—BTB method Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly ...Read more »
-
Rapid determination of seed germination rate—TTC method Seed germination rate refers to the percentage of seeds that germinated in the specified number of days under the most suitable conditions. It is the main basis for determining seed quality and practical value, and it is directly re...Read more »
-
Production and purification of cell culture transfected Wnt ligand [Essence summary] Wnt ligand protein is abundant in vitro, but it is very difficult to purify. This method uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect Wnt11r protein...Read more »
-
Operating Procedures for Embryonic Stem Cell Culture [ different size petri dish,dish petri,disposable sterile petri dish,15cm petri dish,sterile plastic petri dishes ] Maintain ES cells in an undifferentiated state: ES cell culture uses high-sugar medium containing ESGRO (leukemia inhibitory fac...Read more »
-
Use of Petri Dish in Bioassay of Cytokinin [ cheap petri dish,compartment petri dish,sterile petri dishes,petri dish 4 ] Biological identification of cytokinin (radish cotyledon weight gain method) [Principle] Cytokinin has the effect of promoting the enlargement of radish cotyledons (increase in...Read more »
-
Operating Procedures for Air Culture in Blood Storage Room and Blood Matching Room 1. Purpose: To standardize the bacterial culture in the blood storage room and the blood matching room. two. Scope of application: suitable for bacterial culture in the blood storage room and blood matching room of...Read more »
